TABLE 1.
Functional profile of putative NA active-site mutants
| Amino acid substitution | β-Sheet | Avg cell surface expressiona (% of wt) | Avg NAb (% of wt) | Avg HAdb (% of wt) |
|---|---|---|---|---|
| R174L | 1 | 21.0 ± 8.9 | — | — |
| I175E | 1 | 77.8 ± 17.1 | — | — |
| D198Rc | 1 | 89.8 ± 5.0 | — | — |
| K236R | 2 | 66.8 ± 3.6 | — | — |
| R416L | 4 | 27.4 ± 6.4 | — | — |
| R498L | 5 | 116.9 ± 22.6 | 4.2 ± 1.7 | 36.1 ± 3.1 |
| N500D | 5 | 112.6 ± 5.9 | 107.0 ± 27.3 | 94.7 ± 16.2 |
| V502A | 5 | 106.9 ± 17.9 | 107.5 ± 11.8 | 132.5 ± 10.5 |
| Y526L | 6 | 51.5 ± 13.9 | — | — |
| E547Q | 6 | 80.0 ± 16.1 | — | — |
a
Determined by FACS analysis and expressed as a percentage of wt HN. The data are averages of three independent experiments.
b
Expressed as a percentage of wt NDV HN. The data are averages of at least three independent experiments. —, no activity detected.
c
Taken from Deng et al. (7).