FIG. 5.
Summary of mutations made within the reporter-expressing BCoV DI RNA engineered to synthesize mRNA from the mRNA 5 fusion sites (pDrepIS12.7gD). Sites 1, 2, 3, and 4 (in bold) are IS motifs found to function as fusion sites in either the wild type or mutant constructs as noted in the column at the right. Sites identified by parentheses are minor sites. The stop codon for gene 4–1 and the start codon for gene 5 are boxed. The four in-frame stop codons between the DI-RNA ORF and the 12.7-kDa protein ORF are underlined. Sequence numbering refers to base positions in pDrepIS12.7gD. m, mutant.