FIG. 8.
CCR5 and CXCR4 are localized in separate microclusters on human macrophages. (A) Arrowhead shows a homogeneous microcluster of CXCR4 stained with an N-terminal rabbit anti-peptide IgG, and the arrow depicts a separate microaggregate of CCR5 labeled with MAb 2D7; both clusters are localized on a single microvillus. (B) A CCR5 microaggregate is labeled with a C-terminal rabbit antipeptide IgG (arrow), while a CXCR4 microcluster is stained with MAb 12G5 (arrowhead); both are located on the same lamellipodium. Bar = 100 nm.