FIG. 6.
Detection of vector DNA by Southern blot analysis, DNA isolated from liver (A), lung (B), heart (C), and spleen (D) was digested with KpnI and hybridized to a probe from the pol region of Ad5. The blots were exposed to X-ray film for 24 h (A) or 48 h (B to D). Quantitation of the bound probe using an InstantImager instrument is shown in the lower panels of A and D. The counts per minute reported were corrected by subtraction of background determined in adjacent regions of the blot. Samples from mock-treated animals were not above background.