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Estimation of HIV-1 proviral DNA copy number on myDC and pcDC infected in vitro with lymphotropic and macrophage-tropic strains of HIV-1. Limiting-dilution nested PCR to detect HIV-1 provirus in in vitro-infected myDC (a) and pcDC (b) (experiment 5; 9-day infection [Table 1]). PCR was performed on five fivefold dilutions of the DNA template. Lanes 1 to 5 and 6 to 10 on each gel represent assays on IIIB-and Ba-L-infected cultures, respectively.
