TABLE 1.
NV deletion mutant primers
| Primer | Sequence (5′ to 3′) | Positiona (nt) | Polarity |
|---|---|---|---|
| NV Bgl II 225 | TCT GTT AGA TCT GAG CAG AAA ACC AGG CCC | 6030-6052 | sense |
| NV Bgl II 278 | TCT GTT AGA TCT GGC ACC ACC CCA GTT TCA | 6189-6211 | sense |
| NV Bgl II 406 | CGG AGA TCT TTA GGC TAG ATG TGT TGC CTC | 6553-6575 | antisense |
| NV Bgl II 530 | CAA GGA GAT CTT TAT CGG CGC AGA CCA AGC | 6927-6950 | antisense |
| NV Bgl II 514 | CAA GGA GAT CTT TAC TTT AAT TGA TAA AAT | 6880-6899 | antisense |
| NV Bgl II 454 | CGC GTT AGA TCT GGT GAG GCT GCC CTG CTC | 6717-6734 | sense |
| NV Bgl II 466 | CGC GTT AGA TCT ACC GGT CGG AAT CTT GGG | 6753-6770 | sense |
| NV Mfe I 520 | CGG CAA TTG TTA GCT GGC AGT TCC CAC AGG C | 6899-6918 | antisense |
a
NV accession number M87661 in GenBank.