FIG. 4.
SDS-PAGE and Western blot analysis of SA11 4F TR and NTR particles. SA11 4F TLPs grown in the presence (A) and absence (B) of trypsin were purified and treated with increasing concentrations of trypsin as indicated. The proteins were separated by SDS-PAGE, transferred to nitrocellulose, and detected with a hyperimmune anti-SA11 4F TLP mouse serum. The location of the individual proteins is indicated on the right of each panel. The approximate molecular weights for the new bands representing previously uncharacterized cleavage products from viruses exposed to trypsin after purification are indicated. The arrows highlight two new bands representing previously uncharacterized VP5∗ cleavage products of 24K and 22K, and the asterisk highlights a new VP8∗-specific cleavage product of 25K from viruses exposed to trypsin.