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BF cells were infected at a high MOI with wt SV5 or rSV5-V/P N100D and passaged three times over a period of 2 weeks. At 24 h p.i. (a) and after the third passage (b), cells growing on coverslips in the tissue culture dishes were fixed and stained for immunofluorescence using an anti-NP MAb (NP-a).
