FIG. 2.
The growth inhibition phenotype requires the same regions of the vhs polypeptide as mammalian shutoff. Strains YGAL, Yvhs (vhs), YR27 (R27), YN138-HA (N138-HA), YSc243 (Sc243), YS344-HA (S344-HA), and YM384 (M384) were grown to saturation in YNBD, diluted 10−1 to 10−4 in sterile ddH2O, and 2 μl of each dilution was spotted onto YNBD (glucose) and YNBG (galactose) plates. The plates were incubated at 30°C for 3 and 5 days, respectively. The upper portion of the figure displays a linear representation of the 489-residue vhs polypeptide, with the positions of in-frame linker insertion mutations indicated. Regions conserved between the vhs proteins of alphaherpesvirus (conserved regions I, II, III, IV, and A [2, 21]) are presented as shaded and hatched boxes.