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Purification of oligomeric gp140. (A) Superdex-200 A280 elution profile of gp140. The bracketed fractions contain oligomeric gp140, as shown by chemical cross-linking (not shown). These fractions were pooled, concentrated, and used for immunization of macaques. (B) Coomassie blue staining of proteins in the medium of infected cells (Med) and in the purified Env (Olig) from panel A.
