Figure 5.
Lung-infiltrating DCs and macrophages express high levels of IL-12 and NOS2 in 5-LO–deficient hosts infected with M. tuberculosis. Frozen sections of lungs from infected (300 CFU inoculum) WT (A, B, and C) and 5-lo–/– (D, E, and F) mice were double stained with anti-CD11c (A and D) or anti-F4/80 (B, C, E, and F) (green) and with anti–IL-12p40 (A and D), anti-TNF (B and E), or anti-NOS2 (C and F) (red), then counterstained with DAPI (blue). Note the presence of many more CD11c+IL-12p40+ cells and F4/80+NOS2+ cells in the tissue sections from 5-lo–/– animals. The asterisk in A indicates the presence of multinucleated cells at the center of a granuloma. Representative micrographs (magnification, ×63) from 4 animals per group are shown.