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. 2005 Mar 22;387(Pt 1):211–219. doi: 10.1042/BJ20041710

Figure 3. pkd2 overexpression and depletion cause changes in cellular morphology and alter cell wall formation.

Figure 3

(A) Growth of cells containing a plasmid-borne copy of pkd2 under the control of a high-strength inducible promoter (pREP3x) and a medium-strength inducible promoter (pREP41x) on solid medium after 4 days of growth. (B) Appearance of cells overexpressing pkd2 from pREP3x (promoter-induced and -repressed conditions) under bright-field microscopy. (C) Left-hand panel, zymolyase-sensitivity assay for pkd2-depleted cells; (▲) Δpkd2 and pREP41x-pkd2 (promoter-repressed); (■) Δpkd2 and pREP41x-pkd2 (promoter-induced); (●) pkd2 wild-type strain. Right-hand panel, zymolyase-sensitivity assay for pkd2-overexpressed cells; (▲) pkd2 and pREP3X-pkd2 (promoter-induced); (■) pkd2 and pREP3X-pkd2 (promoter-repressed); (●) pkd2 wild-type strain. (D) Cell wall regeneration assay for various strains containing indicated plasmids: i, wild-type pkd2; ii, Δpkd2 and pREP41x-pkd2 (promoter-induced); iii, Δpkd2 and pREP41x-pkd2 (promoter-repressed); iv, pkd2 and pREP3x (promoter-repressed); v, pkd2 and pREP3x (promoter-induced); vi, Δpkd2 and pNR228-pkd2 (fragment); vii, pkd2 and pREP41x-pkd2 (promoter-repressed); viii, pkd2 and pREP41x-pkd2 (promoter-induced); ix, pkd2 and pREP3X-pkd2 (promoter-repressed); x, pkd2 and pREP3x-pkd2 (promoter-induced). (E) Cell wall β-glucan levels as measured by Aniline Blue dye binding for strains with plasmids and conditions as in (D).