Figure 6. Increased protein expression of CAT-1 and eNOS–CAT-1 association in endothelial cells by adenoviral-mediated gene transfer.
(A) BAECs were infected for 24 h with Ad-β-Gal (negative control) or Ad-CAT-1. Lysates were prepared, and equal quantities of lysate proteins from each condition were immunoblotted with anti-CAT-1-ID6 antibody. Three separate experiments gave similar results. Molecular masses are indicated in kDa. (B) Lysates were prepared, and equal quantities of lysate protein from each condition were immunoprecipitated with anti-CAT-1-ID6 antibody. Immunoprecipitates were then immunoblotted with anti-eNOS antibody. Results shown are representative of three experiments.