Figure 4: Distinct pathway and gene signatures in pediatric tonsil Tregs.
Analysis of Treg transcriptional signatures across age within tonsil. A) Pathway analysis graph showing enrichment level of every KEGG 2021 human pathway in the list of differentially expressed genes upregulated in pediatric (aged < 3 years, n = 4) compared to adult (n = 3) tonsil Tregs by −log10 p value vs odds ratio. B) Representative gene set enrichment analysis based on differentially expressed genes in pediatric vs adult tonsil Tregs after removal of cell cycle genes. C) Heatmap depicting row normalized expression of selected Treg genes comparing adult (purple) and pediatric (green) cohorts. * = p<0.05 and log2 fold change >1. NES = normalized enrichment score. GO = gene ontology