TABLE 1.
Construction of site-directed mutations in region I (amino acids 1 to 135)
| Mutant no. | Mutant allele | Steps in constructiona |
|---|---|---|
| 1 | RRE19 | KOS→4→1 |
| 2 | RRE19;SMR65 | KOS→29→26→13→2 |
| 3 | Q11;RSE19 | KOS→4→3 |
| 4 | RSE19 | KOS→4 |
| 5 | Q11;RSE19;H435 | KOS→4→directed Q11 and spontaneous mutagenesis |
| 6 | R22;E25;SMR65 | KOS→29→26→13→9→6 |
| 7 | R19;S22 | KOS→4→7 |
| 8 | R19;E25 | KOS→4→8 |
| 9 | S22;E25;SMR65 | KOS→29→26→13→9 |
| 10 | S22;SMR65 | KOS→29→26→13→9→10 |
| 11 | R22;SMR65 | KOS→29→26→13→9→11 |
| 12 | E25;SMR65 | KOS→29→26→13→9→12 |
| 13 | RSE19;SMR65 | KOS→29→26→13 |
| 14 | RSE19;R68 | KOS→29→14 |
| 15 | RSE19;S65 | KOS→4→15 |
| 16 | RSE19;M66 | KOS→4→15 |
| 17 | RSE19;S65;M66 | KOS→29→26→13→17 |
| 18 | RSE19;SMR65;ET86 | KOS→29→26→21→18 |
| 19 | RSE19;SMR65;E86 | KOS→29→26→13→19 |
| 20 | RSE19;SMR65;T88 | KOS→29→26→13→20 |
| 21 | SMR65;ET86 | KOS→29→26→21 |
| 22 | Q11;SMR65;ET86 | KOS→29→26→21→22 |
| 23 | SMR65;ET86;F131 | KOS→29→26→21→23 |
| 24 | RSE19;T88 | KOS→30→24 |
| 25 | RSE19;R68;T88 | KOS→30→25 |
| 26 | SMR65 | KOS→29→26 |
| 27 | ET86 | KOS→30→27 |
| 28 | R19;ET86 | KOS→30→27→28 |
| 29 | R68 | KOS→29 |
| 30 | T88 | KOS→30 |
a
Steps by which the parent allele was altered by site-directed mutagenesis to yield the mutant allele. Mutant numbers are used.