FIG. 6.
(A) Schematic representation of the poliovirus genome and the primers used to amplify fragments across poliovirus mutant genomes. Three fragments were amplified. Fragment A was amplified from the 5′ terminus with primers E1 and E2, fragment B was amplified from the P1 region with primers D9 and W2074, and fragment C was amplified from the P2-P3 region with primers Po3 and Po4. (B) RT-PCR products were analyzed on a 2.0% agarose gel. RNA was isolated from 150S particles and the empty capsids (80S) of the wild-type poliovirus (PVM) and Win51711-stabilized 2195H.T- and 2195H.R-transfected cell lysates. RT-PCR positive (supplied by the manufacturer) and negative (No templ.) controls are shown. A 100-bp ladder, used as a molecular weight marker, is shown on the left.