FIG. 5.
Cell lysates from wild-type poliovirus (PVM) (A), 2195H.T (B), and 2195H.R (C) cDNA-transfected cells grown in the presence of Win51711 (1 μg/ml) were loaded and analyzed on 15 to 30% sucrose gradients. Lysates were made from PVM (D)-, 2195H.T (E)-, and 2195H.R (F)-transfected cells grown in the absence of Win51711. The lysates were then incubated in the presence (solid circle) or absence (open circle) of Win51711 (10 μg/ml) overnight at 4°C. The lysates were loaded onto 15 to 30% sucrose gradients, and the 150S regions (fractions 33 to 35) were subsequently pooled and immediately rerun on another 15 to 30% sucrose gradient.