FIG. 3.
(A) Equal volumes of [35S]methionine-labeled viral lysates from wild-type poliovirus grown in the presence of the drug hydantoin (open circle) or 2195H.G viral cell lysates grown in the absence of the drug (solid circle) were loaded onto 15 to 30% sucrose gradients and analyzed. Samples were sedimented at 39,000 rpm in a Beckman SW40.1 rotor for 3 h at 4°C, fractionated, and counted. (B) 2195H.G 110S assembly intermediates from gradient A (solid circle) were pooled immediately and rerun along with a wild-type 80S empty capsid marker (open circle) on 15 to 30% sucrose gradients as described above.