FIG. 5.
2D gel electrophoresis. Total cell extracts of NK LCLs stably expressing a control vector (left) or Flag-RK-BARF0 (right) were separated according to their isoelectric points (first dimension) and molecular weights (second dimension). Protein expression was analyzed by immunoblotting by using an anti-Flag MAb (upper and lower) followed by the anti-RK-BARF0 serum (lower). Molecular size markers (expressed in kilodaltons) and the isoelectric point range are given. Arrows and arrowheads indicate the positions of proteins specifically detected by the anti-Flag MAb and by the anti-RK-BARF0 serum, respectively.