Fig. 4. Putative transcriptional trajectories connecting NK cell subpopulations.
a–e, Based on dataset 4a. f–g, Based on datasets 1–4a. a,b, RNA-velocity analysis and pseudotime inference based on velocity analysis in a representative sample. c,d, Confirmation of trajectories and pseudotime analysis using a diffusion-map approach (Destiny analysis of dataset 4a). e, Plot of pseudotime derived from diffusion-map analysis across all NK cell subgroups. f, Monocle-derived trajectory performed on the NK1A, NK1B and NK1C subsets and projected onto the UMAP, colored by clusters. g, Monocle-derived trajectory performed on NK1 subpopulations and projected onto the UMAP colored by pseudotime (inferred by Monocle3).