Fig. 2. GRNs defining conventional and adaptive NK cell fates.
a, UMAP representation highlighting the starting cell (blue) with the lowest value CD56dim signature score and the two terminal cells (orange) as predicted by Palantir. b, UMAP representation of the data from the sorted subsets (two donors) showing the RNA velocity vector field as a stream plot and the inferred pseudotime. c,d, PAGA graph with directionality and transitions from RNA velocity analysis for the sorted subsets (2 donors) (c) and subset-inferred bulk donors (12 donors) stratified based on the presence or absence of adaptive expansion (d). e, Gene trends clustered into five overall trends of expression along pseudotime, showing expression of KLRC2, CD52 and IL32 in both terminal fates (pink, conventional fate; orange, adaptive fate). f, Inferred GRNs where dominant TFs for each trend are highlighted. g, Selection of regulons showing differential expression over pseudotime within the conventional and adaptive fate.