FIG. 2.
Purified soluble receptors. Receptors purified from recombinant baculovirus-infected Sf9 cell supernatants were separated by SDS-PAGE. (A) Purified proteins were electrophoresed on a 12% polyacrylamide gel under reducing and denaturing conditions and then visualized by silver staining. The molecular mass markers are indicated in kilodaltons. (B) Endoglycosidase digestions. HveC(245t) (lanes 1 to 4) and HveC(143t) (lanes 5 to 8) were subjected to digestion with PNGase F (F) or endoglycosidase H (H) or were mock treated (−) prior to electrophoresis on a 16% acrylamide gel under reducing and denaturing conditions. After the Western blotting, proteins were detected with the anti-tetra-His MAb. (C) Native Western blot. Proteins were run under nondenaturing and nonreducing conditions on a 12% polyacrylamide gel and detected with MAb R1.302 (35).