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CCR5-binding ability of gp120 glycoproteins from the adapted virus. The binding of radiolabeled, soluble gp120 glycoproteins to Cf2Th-synCCR5 cells, in the absence or presence of 6 μg of sCD4 per ml, is shown. The gp120 glycoproteins were also incubated with Cf2Th cells not expressing CCR5, in the presence of 6 μg of sCD4 per ml. Equivalent levels of all three gp120 glycoproteins were incubated with the cells, as described in Materials and Methods. w.t., wild type.
