FIG. 4.
Histology and immunostaining of brain sections from an MxA+/+ IFNAR-1−/− and an MxA−/− IFNAR-1−/− mouse. Both types of animals were infected with 105 TCID50 of LACV by the intraperitoneal route. Mice were sacrificed 6 days after infection, and brain sections were prepared. Micrographs show the brain of an IFNAR-1−/− mouse (panels A to D) and that of an MxA+/+ IFNAR-1−/− mouse (panels E to H) immunostained for MxA protein (panels A and E), the nucleocapsid protein of LACV (panels B and F), or GFAP (panels D and H) or stained with hematoxylin and eosin (HE) (panels C and G).