FIG. 7.
Further analysis of ITR deletions by Southern blotting. (A) Locations of the BglI sites in AAV-EF1α-F.IX. The expected fragments obtained by BglI digestion are also shown. (B) Twenty micrograms of liver DNAs from C57BL/6 mice injected with a dose of 2.7 × 1011 particles of AAV-EF1α-F.IX were digested with BglI and hybridized with probe A. The time of sacrifice and the route of administration are indicated above each lane. M, months. (C) Schematic representation of deletion of two BglI sites in the ITR. The 4.4-kb band indicates joining of two BglI fragments of 2.4 and 2.1 kb due to deletions of the ITR sequence involving BglI sites.