FIG. 3.
CD4+ T-cell proliferation in response to stimulation with HPV E7-pulsed autologous irradiated LCL (LCL/E7) in the presence or absence of an HLA class II-specific blocking MAb (L-243, 50 μg/ml). T cells (2 × 104) were cultured in triplicate with LCL/E7 (2 × 104) in a 96-well plate for 72 h. [3H]thymidine (1 μCi/well) was added to each well for at least 16 h of the assay, and proliferation was determined by [3H]thymidine incorporation. Stimulation of CD4+ T cells with irradiated LCL or LCL-DOTAP served as a negative control. Bars: 1, LCL control; 2, LCL-DOTAP control; 3, LCL/E7 plus L243; 4, LCL/E7. The difference in mean proliferation determined by [3H]thymidine incorporation in the presence of LCL/E7 compared to that in the presence of LCL controls is significant at a P value of <0.01, as determined by Student’s t test, for all three patients (PT). Data are given as mean ± standard deviation.