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Nucleotide sequence of the region of the F gene that specifies the protease cleavage site of the F0 protein of strains NDFL (top) and NDFLtag (bottom). DNA fragments containing the relevant regions were generated by RT-PCR and sequenced by using a PRISM kit (Perkin-Elmer) and an Applied Biosystems ABI310 automatic sequencer. The three nucleotide replacements, which were introduced in pNDFLtag by means of PCR mutagenesis, are boxed (cf. Fig. 2C).
