FIG. 6.
Time course of MCMV DNA synthesis. Quiescent NIH 3T3 cells were infected with MCMV (MOI, 5 PFU/cell). Total genomic DNA was purified at the indicated times and immobilized on a hybridization membrane by a dot blot apparatus. The same filter was sequentially hybridized with a 32P-labeled viral probe (a 1,104-bp AvaI-XbaI fragment in the fourth exon of the MCMV IE1 gene) and a cell DNA probe (full-length murine G3PDH cDNA). The hybridization signals were quantitated with the Bio-Rad image analysis system and normalized to the differences in cell DNA. Values are the means of two separate determinations.