FIG. 3.
M3 protein blockade of murine chemokine activity. Shown are changes in the relative fluorescence of Fura II-AM-loaded cells, which monitors intracellular Ca2+ concentration. Test substances were added where indicated at 100 nM, except for fMLF and ATP, which were added at 1 μM. H2M is a control protein which was purified in a similar manner to M3; mFkn, m-fractalkine. Each tracing corresponds to the target indicated to the left of the row in which it is found. Each row of tracings corresponds to the same target chemokine. Neutrophils and macrophages represent cells harvested from C57BL/6 mice 3 and 72 h after instillation of thioglycolate. CX3CR1 represents a human embryonic kidney 293 cell line expressing human CX3CR1. Data are representative of at least two separate experiments for each chemokine. (a) KC; (b) mMIP-1α; (c) fMLF and mMIP-1α; (d) mMCP-1 and SDF-1; (e) m-fractalkine.