FIG. 2.
JSRV LTR activity in cell lines. The pJS21-luc plasmid was transfected into a series of murine, ovine, and human cell lines. Luciferase activities of pJS21-luc relative to the activity of pGL3, a reporter plasmid driven by the SV40 promoter-enhancer are shown. The activity of pGL3 in each cell line was set at 100%. All transfections included cotransfection with a second reporter plasmid driving a different reporter gene to control for transfection efficiency in different experiments (see Materials and Methods). At least six replicates were tested for each cell line, and the standard error of the mean is indicated by the error bars.