TABLE 5.
Vector titers for the pHS series of vector genomes in the presence or absence of Rev/RREa
| Vector genome | Titer (TU/ml) for:
|
|
|---|---|---|
| pSYNGP | PSYNGP + Revb | |
| pH6nZ | 6.5 × 105 | 6.9 × 105 |
| pH6.1nZ | 1.5 × 104 | 1.7 × 104 |
| pH6.1nZR | 2.3 × 103 | 1.6 × 105 |
| pHS1nZ | 8.5 × 103 | 8.4 × 103 |
| pHS1nZR | 8.1 × 103 | 8.8 × 104 |
| pHS3nZ | 1.2 × 105 | 1.4 × 105 |
| pHS3nZR | 2.5 × 103 | 4.8 × 105 |
| pHS7nZ | 7.3 × 103 | 7.0 × 103 |
| pHS7nZR | 3.4 × 103 | 5.8 × 103 |
a
The retroviral vector was generated as described in Materials and Methods. Five micrograms of vector genome, 5 μg of pSYNGP, and 2.5 μg of pHCMVG were used, and titers were determined in 293T cells. Experiments were performed at least twice, and the variation between experiments was less than 15%.
b
Rev was provided from pCMV-Rev (1 μg). Note that pH6nZ expresses Rev and contains the RRE. None of the pHS genomes expresses Rev, and only pHS1nZR, pHS3nZR, pHS7nZR, and pH6.1nZR contain the RRE. Gag-Pol expression from pSYNGP is Rev independent.