TABLE 3.
Vector titers from genomes with or without Rev/RREa
| Gag-Pol expression vector | Vector genome | Rev expression/ plasmid | Titer (TU/ml of viral stock ± SD) |
|---|---|---|---|
| pGP-RRE3 | pH6nZ | Yes/pH6Z | (8.0 ± 0.2) × 105 |
| pGP-RRE3 | pH6.1nZ | No | (2.1 ± 0.3) × 103 |
| pGP-RRE3 | pH6.1nZ | Yes/pCMV-Rev | (4.1 ± 0.3) × 104 |
| pGP-RRE3 | pH6.2nZ | No | (1.7 ± 0.4) × 103 |
| pGP-RRE3 | pH6.2nZ | Yes/pCMV-Rev | (5.6 ± 0.3) × 104 |
| pSYNGP | pH6nZ | Yes/pH6Z | (7.8 ± 0.2) × 105 |
| pSYNGP | pH6.1nZ | No | (5.4 ± 0.3) × 104 |
| pSYNGP | pH6.1nZ | Yes/pCMV-Rev | (5.2 ± 0.4) × 104 |
| pSYNGP | pH6.2nZ | No | (5.0 ± 0.2) × 104 |
| pSYNGP | pH6.2nZ | Yes/pCMV-Rev | (5.5 ± 0.3) × 104 |
a
The retroviral vector was generated as described in Materials and Methods. Titers were determined in 293T cells.