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RPMS1 inhibition of transcription induced by EBNA-2 or NotchIC on reporter plasmids pJH26A (8 × CBF1-Luciferase) or pPDL83A (4 × CpE2RE-CAT). All transfections received 1 μg of reporter plasmid and 1 μg of either EBNA-2 or Notch expression vector, as indicated. Additionally, 5 μg of plasmids expressing RPMS1, RPMS1(34-103), or RPMS1(SR) was cotransfected as shown. Results are given as a mean of three experiments, normalized on the values for EBNA-2 or NotchIC activation.
