Figure 6.
Epigenetic inactivation of DFNA5 in primary gastric cancers. (A) The methylation status of DFNA5 was determined by combined bisulfite restriction analysis (COBRA): N, normal tissues; T, tumors. (B) Bisulfite sequencing of DFNA5. Each square represents a CpG site. The distance from the transcription start site is shown above, as is the restriction enzyme site used for COBRA: □, unmethylated alleles; ▪, methylated alleles. (C) Correlation between expression and methylation of DFNA5. Expression levels of DFNA5 were determined using the ΔΔCT method with ABI7900.