FIG. 5.
Interaction of SicA with SipB and SipC. Whole-cell lysates of the S. enterica serovar Typhimurium strain SB319 which expresses a functional, chromosomally encoded, M45-epitope-tagged form of SicA were immunoprecipitated with antibodies directed to SipB, SipC, or a preimmune serum (Pre), as described in Materials and Methods. Proteins that bound to the beads (P) or that remained in the supernatant (S) were probed by Western immunoblotting for the presence of M45-SicA with a monoclonal antibody directed to the M45 epitope.