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Absence of SicA results in decreased SipB and SipC stability. Wild-type S. enterica serovar Typhimurium and the isogenic sicA mutant strain SB221 were pulse-labeled with [35S]methionine for 2 min and chased with cold methionine for 60 min. At the indicated time points, samples were removed, immunoprecipitated with anti-SipB and anti-SipC polyclonal antibodies, and run on an SDS-PAGE gel. Labeled proteins were visualized by fluorography.
