FIG. 3.
Schematic representation of RNA fragments of the HAV 5′NCR used as competitor RNAs in mobility shift assays with 32P-labeled PV stem-loop IV RNA and rPCBP2. Open bars represent sequences of the 5′NCR comprising nucleotides indicated below each fragment. The dotted lines indicate the extent of deletions in the 5′NCR. The vertical lines indicate the borders of the predicted stem-loop structures I to V and the pyrimidine-rich tracts at the 5′ (pY1) and 3′ (pY2) ends of the 5′NCR (8, 38) as depicted above the diagram of the RNA fragments. +, (+), or − at the left indicates whether the particular RNA fragment competes, competes weakly, or does not compete with PV stem-loop IV RNA for binding of rPCBP2.