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Intranuclear distribution of unspliced HIV-1 env mRNA in the presence or absence of Rev expression. HeLa CMV*/Rev/pgEnv Hygro cells were grown for 5 days in the absence (A and C) or presence (B and D) of Rev expression, fixed, hybridized to DIG-labeled antisense RNA probe specific to HIV-1 unspliced env RNA, and immunostained with FITC-conjugated anti-DIG antibody (green). In all panels, cells were stained with anti-SC35 antibody (red).
