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. 1986 Apr;21(3):205–208. doi: 10.1007/BF00199363

Distribution of intact and Fab1 2 fragments of anti-human chorionic gonadotrophin antibodies in nude mice bearing human choriocarcinoma xenografts

Frances Searle 1,✉, Theresa Adam 1, Joan A Boden 1
PMCID: PMC11041094  PMID: 3698059

Abstract

A direct comparative study of the distribution of intact and Fab1 2 fragments of mouse monoclonal anti-human chorionic gonadotrophin antibody, W14A, in nude mice bearing a human choriocarcinoma xenograft, indicates that the tumour:blood ratio is generally improved with fragments. Some parameters which may determine the distribution are outlined.

Keywords: Cancer Research, Nude Mouse, Chorionic Gonadotrophin, Fab12 Fragment, Choriocarcinoma

References

  • 1.Arend WP, Teller DC, Mannik M. Molecular composition and sedimentation characteristics of soluble ag — ab complexes. Biochemistry. 1972;11:4063. doi: 10.1021/bi00772a008. [DOI] [PubMed] [Google Scholar]
  • 2.Begent RHJ, Bagshawe KD. Clinical applications of radioimmunolocalisation. In: Baldwin RW, editor. Monoclonal antibodies for tumour detection and drug targeting. London: Academic Press; 1985. [Google Scholar]
  • 3.Brennan FM, Grace SA, Elson CJ. Preparation of covalent IgG complexes of defined size and their clearance from the circulation of mice. J Immunol Meth. 1983;56:149. doi: 10.1016/0022-1759(83)90406-4. [DOI] [PubMed] [Google Scholar]
  • 4.Buchegger F, Haskell CM, Schreyer M, Scazziga BR, Randir S, Carrel S, Mach J-P. Radiolabelled fragments of monoclonal antibodies against carcinoembryonic antigen for localisation of human colon carcinoma grafted into nude mice. J Exp Med. 1983;158:413. doi: 10.1084/jem.158.2.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Burchiel SW, Burke D, Breslow K, Austin R, Reed K, Rhodes B. Biodistribution of radiolabelled antibodies and their fragments: an immunopharmacologic approach to in vivo tumour imaging. Proc West Pharmacol Soc. 1982;25:405. [PubMed] [Google Scholar]
  • 6.Cavalieri RR, Rapaport B. Impaired peripheral conversion of thyroxine to triiodothyronine. Ann Rev Med. 1977;28:57. doi: 10.1146/annurev.me.28.020177.000421. [DOI] [PubMed] [Google Scholar]
  • 7.Colcher D, Zalutsky M, Kaplan W, Kufe D, Austin F, Schlom J. Radioimmunolocalisation of human mammary tumours in athymic mice by a monoclonal antibody. Cancer Res. 1983;43:736. [PubMed] [Google Scholar]
  • 8.Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:70. doi: 10.1016/0003-9861(59)90090-6. [DOI] [PubMed] [Google Scholar]
  • 9.Folkers K, Shin S, Kappel M. Structurally restricted conjugates of the subunit of human chorionic gonadotrophin to produce antisera. Biochem Inter. 1983;7(6):761. [PubMed] [Google Scholar]
  • 10.Herlyn D, Powe J, Alavi A, Mattis JA, Herlyn M, Ernst C, Vaum R, Koprowski H. Radioimmunodetection of human tumour xenografts by monoclonal antibodies. Cancer Res. 1983;43:2731. [PubMed] [Google Scholar]
  • 11.Johnson PM, Brown PM. Review Article: Fc receptors in the human placenta. Placenta. 1981;2:355. doi: 10.1016/s0143-4004(81)80031-8. [DOI] [PubMed] [Google Scholar]
  • 12.Mannik M, Arend WP, Hall AP, Gilliland BC. Studies on antigen-ab complexes: elimination of soluble complexes from rabbit circulation. J Exp Med. 1971;133:713. doi: 10.1084/jem.133.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Morgan FJ, Canfield RE. Nature of the subunits of human chorionic gonadotrophin. Endocrinology. 1971;88:1045. doi: 10.1210/endo-88-4-1045. [DOI] [PubMed] [Google Scholar]
  • 14.Morgan FJ, Birken S, Canfield RE. The amino acid sequence of human chorionic gonadotrophin. J Biol Chem. 1975;250:5247. [PubMed] [Google Scholar]
  • 15.Mason AW, Williams AF. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980;187:1. doi: 10.1042/bj1870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Nisonoff A, Hopper JE, Spring SB. The antibody molecule. New York: Academic Press; 1975. p. 235. [Google Scholar]
  • 17.Nisonoff A, Hopper JE, Spring SB, editors. The Antibody Molecule. New York: Academic Press; 1975. p. 325. [Google Scholar]
  • 18.Ogston AG, Preston BN, Wells JD. The transport of compact particles through solutions of chain-polymers. Proc Soc Lond A. 1973;333:297. [Google Scholar]
  • 19.Poste G. Liposome targeting in vivo: problems and opportunities. Biol Cell. 1983;47:19. [Google Scholar]
  • 20.Quinones J, Mizejewski G, Beierwaltes WH. Choriocarcinoma scanning using radiolabelled antibody to chorionic gonadotrophin. J Nucl Med. 1971;12(2):69. [PubMed] [Google Scholar]
  • 21.Scherphof G. In: Liposomes in biological systems. Gregoriades G, Allison AC, editors. New York: John Wiley & Co; 1980. p. 179. [Google Scholar]
  • 22.Searle F, Boden J, Lewis JCM, Bagshawe KD. A human choriocarcinoma xenograft in nude mice; a model for the study of antibody localisation. Br J Cancer. 1981;44:137. doi: 10.1038/bjc.1981.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Searle F, Partridge CS, Kardana A, Green AJ, Buckley RG, Begent RHJ, Rawlins GA. Preparation and properties of a mouse monoclonal antibody (W14A) to human chorionic gonadotrophin. Int J Cancer. 1984;33:429. doi: 10.1002/ijc.2910330402. [DOI] [PubMed] [Google Scholar]
  • 24.Sedlacek HH, Gronski P, Hofstaetter T, Kanzy EJ, Schorlemmer HU, Seiter FR. The biological properties of immunoglobulin G and its split products [F(ab1)2 and Fab] Klin Wochenschr. 1983;61:723. doi: 10.1007/BF01497399. [DOI] [PubMed] [Google Scholar]
  • 25.Silverstein SC, Steinman RM, Cohn ZA. Endocytosis. Ann Rev Biochem. 1977;46:669. doi: 10.1146/annurev.bi.46.070177.003321. [DOI] [PubMed] [Google Scholar]
  • 26.Tomlinson E, McVie JG (1983) New directions in cancer chemotherapy 2. Targeting with microspheres. Pharmacy Inter 281
  • 27.Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA. 1979;76:4350. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Turner MW. In: Structure and function of antibodies. Glynn LE, Steward MW, editors. New York: J Wiley & Sons; 1981. p. 38. [Google Scholar]
  • 29.Vaupel P, Muller-Kleiser W. Interstitieller Raum und Mikromilieu in malignen tumoren. Mikrozirk Forsch Klin. 1983;20:78. [Google Scholar]
  • 30.Wataya Y, Santi DV. Thymidylate synthetase catalysed dehalogenation of 5-bromo and 5-iodo-21-deoxyuridylate. Biochem Biophys Res Commun. 1975;67:818. doi: 10.1016/0006-291x(75)90886-4. [DOI] [PubMed] [Google Scholar]
  • 31.Widder KJ, Senyei AE, Sears B. Experimental methods in cancer therapeutics. J Pharm Sci. 1982;71:379. doi: 10.1002/jps.2600710403. [DOI] [PubMed] [Google Scholar]

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