Fig. 4.
MART-1-specific CTLs, induced by MPLA/IFNγ DCs specifically and effectively lyse target cells. a 51Cr-loaded T2 cells either loaded with Her2 or MART-1 peptide were incubated in different ratio’s with CTLs in triplicate, 7 days after the third stimulation (day 24). Percentage of lysis by representative cultures from one experiment out of five experiments is shown. Mean ± SD of triplicates is depicted. MPLA/IFNγ DCs induced CTL culture A was 40% MART-1 TM positive and culture B was 20% MART-1 TM positive. CC-DC-induced CTL culture was 4.4% MART-1 TM positive. b The CTLs of the same cultures were also incubated with 51Cr-loaded MEL-AKR (MART-1+, HLA-A2+) and MEL-JKO (MART-1+, HLA-A2−) in different ratios. Percentage lysis is depicted as mean ± SD