Fig. 2.
Construction and characterization of ITIP. a Schematic representation of ITIP and its amino acid sequences. b Dimensional conformation for ITIP. The molecular modeling calculation of ITIP was carried out using the Insight II software package. ITIP formed a chemokine-like conformation, including N-terminus, N-loop, three-stranded β-sheet, and C-terminal α-helix. c ITIP was purified using a nickel-chelating column and assayed by Tricine-SDS-PAGE. d The construction of ITIP was confirmed by western blotting with Abs against N-terminal (lane 1) or C-terminal (lane 2) of CXCL11, N-terminal (lane 3) or C-terminal (lane 4) of CXCL10. ITIP polyclonal Ab was prepared and tested by western blotting against CXCL11 (lane 5), CXCL10 (lane 6) and ITIP (lane 7) proteins