Fig. 1.
Tumor M-MDSCs were more suppressive than splenic M-MDSCs. a Gating strategy to identify tumor M-MDSC. It is the same with splenic M-MDSC. b Quantitative RT-PCR analysis of suppression-associated genes Arg1, NOS2 and PD-L1. c IL-6, IL-10 and TGF-β expression of tumor M-MDSCs and splenic M-MDSCs. d CD3+ T cells were isolated by MACS and cocultured with tumor M-MDSCs and splenic M-MDSCs, respectively, in a 48-well plate precoated with anti-CD3 (5 µg/mL) and anti-CD28 (2 µg/mL). CD69 and IFN-γ expression of CD3+T cells were measured 24 h later and proliferation of T cells labeled with CFSE was measured 72 h later (M-MDSC: T ratio = 1:4). Data are pooled from three independent experiments each with 3–5 mice and expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001