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Confocal microscopy analysis of the colocalization of Tax and CREB-2 in vivo. Cos7 cells were cotransfected with pSG-Tax and pEGFP-CREB-2263–351. Analysis of the red (A), green (B), and merged (C) fluorescence was performed with a Bio-Rad MRC 1024 confocal microscope. The Tax protein was detected by using culture supernatant of the anti-Tax 168A51-42 hybridoma and goat anti-mouse immunoglobulin G antibody coupled to rhodamine (Pierce).
