FIG. 5.
Endo H treatment of envelope glycoproteins. Cells were radiolabeled and chased for 1 h (lanes 1H), 2 h (lanes 2H), or 3 h (lanes 3H). Glycoproteins were immunoprecipitated from the resulting cell lysates and split in half for either endo H digestion (lanes +) or mock treatment (lanes −). Results for the FLAG and ERRS mutants are shown in the top and bottom panels, respectively. gp160S indicates glycoprotein precursor which was sensitive to endo H digestion. gp120R and gp160R indicate processed glycoprotein and glycoprotein precursor, respectively, which was resistant to endo H digestion.