FIG. 5.
Competitive inhibition of Rev function. COS cell cultures were cotransfected with the HXB-2-derived Rev-deficient proviral DNA HIV-1Δrev, pBC12/RSV/SEAP (internal control vector), and pBC12/CMV/IL-2 (negative), pcRev (positive), or pcRev plus a 10-fold excess of expression vector encoding wild-type (WT) Rev, RevSLT26, RevSLT40, or RevM10. Total input DNA was maintained at a constant level by inclusion of parental vector pBC12/CMV. All p24 Gag antigen values were adjusted for transfection efficiency by determining the level of SEAP (5) in each culture and were corrected for background (mock) activity. Error bars represent the standard deviations of three independent experiments.