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RT-PCR analysis of EBV latent gene expression in virus-infected epithelial cells. LCL, B-lymphoblastoid cell lines immortalized with rEBV, was used as a positive control for detection of LMP2A, LMP2B, BARF0, and Cp- or Wp-initiated EBNA mRNAs. rEBV-infected Akata− (tk+) cells were used as a positive control for detection of Qp-initiated EBNA mRNA. HeLa cells served as a negative control. Labels on the lanes are explained in the legend to Fig. 4.
