FIG. 7.
E2 proteins were detected in COS-7 cells infected with pPAVAkzE2-TA viruses by indirect immunofluorescence with the B201 E2-specific antibody. (a and b) SV40-infected COS-7 cells used as a control; (c and d) cells infected with pPAVAkzE2-TA; (e and f) cells infected with pPAVAkzE2-TAΔ41–120; (g and h) cells infected with pPAVAkzE2-TAΔ51–120. In panels a, c, e, and g, cellular DNA was detected by propidium iodide (PI) staining. In panels b, d, f, and h, FITC-labeled E2 protein is detected in the same fields of cells. Mitotic cells are indicated with white arrowheads in the propidium iodide-stained images.