FIG. 5.
DNase I HS analysis of LCR mutations in MEL cells. Cells were digested with increasing concentrations of DNase I, and DNA was cut with PvuII and analyzed by hybridization against probes 5′ of 5′HS5 (left, EB) and 3′ of 5′HS1 (right, RN). The HSs detected in the different lines are indicated. Note that because of the deletions, the size of the PvuII band and the locations of the HSs differ between the lines. The fragments which can be observed in the different mutations with the two probes are indicated to the right, with P denoting PvuII sites. 5′HS3 is located so close to a PvuII site that it cannot be distinguished from the PvuII band by this probing strategy. The 5-neo-4 insertion leads to the insertion of a PvuII site between 5′HS5 and 5′HS4. All data shown are from one filter that had been hybridized sequentially to the EB and the RN probes. wt, wild type.