Fig 1. Processing pipeline for proteomics analysis of postmortem samples from the lateral orbitofrontal cortex.
A Coronal section of human cortex showing Brodmann’s Areas (BA) including the lateral OFC (Orbital Prefrontal (OP) 13) that was acquired for proteomics analysis. B Twenty-eight samples from age- and sex-matched controls and AUD cases were digested and labeled using two 16-plex TMT kits before protein identification and quantitation (n = 7 condition/sex). Data were then normalized to control for batch effects across different mass spectrometry runs, and differential expression (DE), rank-rank hypergeometric overlap (RRHO), gene ontology (GO) enrichment, and machine learning (ML) analyses were completed.