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Mutation of a putative ERE affects V3 splicing. RT-PCR analysis of splicing from constructs in which the purine-rich stretch of V3 was replaced by pyrimidines is shown. Lane 1, unmutated pV3; lanes 2 and 3, mutation of 5′ and 3′ halves, respectively; lane 4, double mutant. Fragment sizes are indicated in base pairs.
